The cursor will turn red, and then click on the center of the solvent peak. To calibrate, zoom in to the general area of the peak you want to calibrate.For calibrating 1H data, calibrate to the TMS/DSS peak (if using an internal standard), or the residual solvent peak.Try the Indirect Referencing Calculator on Google Docs! Indirect Referencing in Topspinįirst, acquire both a 1H and X nucleus 1D spectrum, as normal. Scroll down for indirect referencing in MNova. The following instructions are for indirect referencing in Topspin (NEO400, AVII600, AVIII600, AV400). Sometimes, internal standards will react with your compound, or the correct internal standard compound can be difficult to obtain. Indirect referencing can provide accurate referencing without having to add an internal standard. Either an internal standard or indirect referencing can be used. When acquiring X nucleus spectrum such as 11B, 31P or 29Si, the spectrum must be referenced to something other than the solvent, since the solvent likely does not contain the nucleus.
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